The rapid foam of preparative chromatography is “flash chromatography”. Flash column chromatography is usually carried out with a mixture of two solvents, with a polar and a nonpolar component. A "State of Art" method of chromatography is called Flash chromatography when solvent is force down to the column with the external air pressure to isolate desired organic compounds. The solvent used for the flash column chromatography depends on the retention factor, retention factor is the calculated by measuring the distance traveled by sample and the solvent. The common solvent used Ether/Petroleum Ether, Ether/Hexane, Ether/Pentane, Ethyl Acetate / Hexane, Methanol/Dichloromethane and many more. The advantages of using flash column chromatography over conventional column chromatography is that It is less solvent usage, greater flexibility and faster. The percentage of yield obtain is higher due to the fine particle of silica gel and aluminium oxide. Flash chromatography methods for chemical compounds purifications, medical chemistry, laboratories research , natural products separation.

 

The media use in flash column chromatography, is silica gel 200-400, 230-400 and 400-800 mesh practical size powder in column and then after solvent is loaded in to the column with medium air pressure of solvents which is optimized particular rapid separation and purification of targeted molecules.  In flash column 40-63 microns are used for cutting-edge applications such as drug discovery, proteins and vitamins purification, chemical research and also use in other pharmaceutical research applications. We also provides aluminium oxide powder with different mesh sizes – 100-200 mesh, 100-300 mesh and 200-400 mesh sizes for flash column chromatography.

 

Adsorbent particle size affects how the solvent flows through the column. Finer particles (higher mesh values) are used for flash chromatography; coarser particles (lower mesh values) are used for gravity chromatography. For example, silica gel 32-63 microns and 60-210 microns is used for gravity columns and 40-63 microns for flash columns.

 

Chromatography Adsorption Mesh Sizes:

 

Silica Gel (SiO2) and Alumina (Al2O3) are two adsorbents commonly used by the organic chemist for column chromatography. These adsorbents are available in different grade "mesh" size, as maintained by a number on the bottle label: “silica gel 60” "silica gel 30-70" "silica gel 60-120" "silica gel 60-200" "silica gel 70-230" "silica gel 100-200" "silica gel 200-400" "silica gel 230-400" or "silica gel 400-800" is a couple examples. This number represents to the mesh of the sieve used to size the silica, particularly the variety of gaps in the mesh or sieve through which the crude silica particle mixture is passed in the manufacturing process.